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Electrocortical spectral analysis and fractal methods for assessing the effects of unilateral brain injury on rat cerebellum

L D Martac ́, S Sekulic ́, M Cvijanovic ́


We used electrocortical spectral analysis and fractal methods for assessing the effects of unilateral, single brain injury on cerebellum. Cerebellar electrocortical activity was recorded in control state (before the injury) and after a single brain injury of the cerebellar cortex in anesthetized rats. We noticed that the mean power in gamma high-frequency domain (32-128 Hz) of the cerebellum, was increased after the first brain injury, while after a two-week recovery, it was larger than before the injury. The unilateral brain injury induced a permanent increase of the mild gamma activity in both the left and the right side of cerebellum cortex, but there was no further increase after the lesion was repeated. Our recent electrophysiological study on the cerebellum (Culic et al., 2005) suggested that the mean power spectra of the cerebellar cortical activity in the gamma frequency range might be the indicator of acute single focal brain injury. However, there is insufficient information on the effects of the repeated brain injury on the cerebellar electrocortical activity and morphology. There was no significant difference between the absolute and the relative mean power of the left and the right paravermal cortical activity (before, as well as, after the injury), in each of the animals tested afterwards, but there were differences between the left and the right side of cerebellum in experimental animals. Repeated injury of the cerebellar cortical areas, is strengthened by morphological changes in the cerebellar hemisphere, and shows a decrease in delta and an increase in gamma range.


cerebellum,lesion,spectral and fractal analysis

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Bischoff-Grethe A., Ivry R.B. and Grafton S.T. Cerebellar involment in response reassignment rather than attention. J.Neurosci. 22(2):546-553,2002.

Borgers C., Epstein S., Kopell N.J. Background gamma rhythmicity and attention in cortical local circuit: A computational study. PNAS, 102: 7002-7007, 2005.

Bower J.M. Control of sensory data acquisition. Int.Rev.Neurobiol. 41:492-513,1997.

Bower J.M. Is the cerebellum sensory for motors sake, or motor for sensorys sake: the view from the whiskers of a rat? Prog. Brain Res .483-516,1997.

Buzsaki G.,Wang XJ. Mechanism of gamma oscillations. Annu.Rev. Neurosci.,35:203-225, 2012.

Choi K. G., Cho E.K., Chae S. H., Kim E.S., Kim J.S. Spectral and bispectral EEG analysis in acute unilateral ischemic stroke patients. Neurol. Psychiatry Brain Res., 7: 9-14, 1999.

Culic M., Martac Blanusa L., Grbic G., Spasic S., Jankovic B., Kalauzi A. Spectral analysis of cerebellar activity after acute brain injury in anesthetized rats. Acta Neurobiol. Exp.,65: 11-17, 2005.

Ćulić, M., Grbić, G., Martać Blanuša, Lj., Spasić, S., Janković, B, Ranković, R., 2003. Slow and fast oscillations in the activity of parietal cortex after brain injury. In: Gantchev, N. (Ed.), From Basic Motor Control to Functional Recovery III, St. Kliment Ohridski University Press, Sofia, Bulgaria, pp. 41-45.

Ćulić M., Martać Lj., Grbić G., Spasić S., Kalauzi A., Janković B. Occurrence of gamma (33 - 128 Hz) activity in the cerebellum of anesthetized rats. 3rd FENS, 13-17 July 2002, Paris-France, Abstract A192.1, 2002.

D'Angelo E., Koekkoek S.K.E., Lombardo P., Solinas S., Ros E.,Garrido J.,Schonewille M., De Zeeuw. Timing in the cerebellum: oscillations and resonance in the granular layer. Neuroscience, 162: 805-815, 2009.

Dawson L. A., Djali S., Gonzales C., Vinegra M. A., Zaleska M. M. Characterization of transient focal ischemia-induced increases in extracellular glutamate and aspartate in spontaneously hypertensive rats. Brain Res. Bull., 53: 767-776, 2000.

De Solages C., Szapiro G., Brunel N., Hakim V., Isope P., Buisseret P., Rousseau C., Barbour B., Lena C. High frequency organization and synchronz of activity in the Purkinje cell layer of the cerebellum. Neuron, 58(5):775-788, 2008.

De Ford M. S., Wilson M. S., Rice A. C., Clausen T., Rice L. K., Barabnova A., Bullock R., Hamm R. J., Repeated mild brain injuries result in cognitive impairment in B6C3F1 mice. J. Neurotrama, 19: 427-438, 2002.

Finnie J. W., Blumberg P. C. Animal models. Traumatic brain injury. Vet. Pathol., 39: 679-689,2002.

De Zeeuw C., Hoebeek FE., Schonewille M. Causes and consequences of oscillations in cerebellar cortex. Neuron, 58(5): 655-658, 2008.

Gaetz M. The neurophysiology of brain injury. Cl. Neurophysiol., 115: 4-18, 2004.

Gianetti S., Molinari M. Cerebellar input to the posterior parietal cortex in the rat. Brain Res. Bull., 58: 481-489, 2002.

Hartmann M.J. and Bower J.A. Oscillatory activity in the cerebellar hemispheres of unrestrained rats. J Neurophys. 80:1598-1604,1998.

Higuchi T. Approach to an irregular time series on the basis of the fractal theory. Physica D., 31: 277-83, 1998.

Juhasz C., Kamondi A., Szirmai I. Spectra EEG analysis following hemispheric stroke. Acta Neurol. Scand., 96: 397-400, 1997.

Kalauzi A., Spasić S., Martać Lj., Grbić G., Ćulić M. Occurrence of gamma (33 - 128 Hz) activity in rat somatosensory cortex. 3rd FENS, 13-17 July 2002, Paris-France, Abstract A024.17, 2002.

Martać Lj., Ćulić M., Grbić G., Kalauzi A., Jovanović A. Occurrence of gamma (33 - 128 Hz) activity in the dorsal hippocampus of anesthetized rats. 3rd FENS, 13-17 July 2002, Paris-France, Abstract A119.2, 2002.

Martać Lj., Ćulić M., Grbić G., Spasić S. Slow and fast oscillations in the activity of left and right cerebellar cortex after brain injury in rats. 10th Young Neuroscientists Meeting, 29 May 2003, Trieste-Italy, Abstract 12, 2003.

Milenkovic I., Filipovic R., Nedeljkovic N., Pekovic S., Culic M., Rakic L., Stojiljkovic M. Spatio-temporal changes in neurofilament proteins immunoreactivity following kainate-induced cerebellar lesion in rats. Cell. Mol. Neurobiol., 24 : 367-378, 2004.

Ordek G., Proddutur A., Santhakumar V., Pfister B.J., Sahin M. Electrophysiological monitoring of injury progression in the rat cerebellar cortex. Front Syst Neurosci., 8: 197, 2014.

Petrosini L., Molinari M., Dell'Anna M E. Cerebellar contribution to spatial event processing: Morris water maze and T-maze. Eur J Neurosci., 8:1882–1896,1996. Cerebellar contribution to spatial event processing: Morris water maze and T-maze

Saini S., DeStefano N., Smith S., Guidi L. , Amato M.P., Federico A., Matthews P. M. Altered cerebellar functional connectivity mediates potential adaptive plasticity in patients with multiple sclerosis. J. Neurol. Neurosurg. Psychiatry, 75: 840-846, 2004.

Sasaki K., Bower J.M., Llinas R. Multiple Purkinje cell recordings in rodent cerebellar cortex. European Journal of Neuroscience, Vol1(6):572-586, 1989.

Schutter E.D. Cerebellar long term depression might normalize excitation of Purkinje cells:a hypothesis. Trends Neurosci.,18: 291-295, 1995.

Spasić S., Ćulić M., Grbić G., Martać Lj., Sekulić S., Mutavdžić D. Spectral and fractal analysis of cerebellar activity after single and repeated brain injury. Bull Math Biol.,70(4): 1235-1249, 2008.

Spasić, S., Kalauzi, A., Grbić, G., Martać, Lj., Ćulić, M. Fractal analysis of rat brain activity after injury. Med. Biol. Eng. Comput., 43: 345-348, 2005.

Veeranna K. T., Boland B., Odrljin T., Mohan P., Basavarajappa B. S., Peterhoff C., Cataldo A., Rudnicki A., Amin N., Li B. S., Pant H. C., Hungund B. L., Arancio O., Nixon R. A. Calpain mediates calcium-induced activation of the Erk 1, 2 MAPK pathway and cytoskeletal phosphorilation in neurons. Am. J. Pathology, 165: 795-805, 2004.



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